Well we were in the 4th row so I could see Jon pretty well and I didn't notice any signs of a bad mood.

In fact, he seemed to be in a better mood than several other times I've seen them. You say he only smiled 5 times - I saw a lot more than that. Also, it was the first time I have seen him point out song requests on signs and then actually play them.
And about the f*** you incident... he wasn't pissed off! When he saw the sign he flipped her off and mouthed the words f.uck you and then LAUGHED. It was done jokingly!